The aim of the study was to establish the animal model of qi deficiency and blood stasis syndrome for drug reasearch. Rats were consecutively fed with high fat diet and then treated with exhaustive swimming exercising. The external signs of rats were observed and twenty-six biochemical parameters were detected

the parameters included energy metabolisms

oxidative stress

lipid metabolism

inflammatory cytokines

immune response

myocardial enzyme

endothelial function and clotting time etc. The results showed that the model rats were inactive

weak and appeared in lethargy

and showed a dark purple tongue

reflecting the clinical characteristics of Qi deficiency and blood stasis syndrome. Compared with the normal group

the model rats showed significant changes in the biochemical indexes mentioned above. All results together indicated that high fat diet combined with the continual exhausting swimming could cause the disorders of energy metabolisms

oxidative stress

and so on. Such disorders are closely associated with the generation and development of Qi deficiency and blood stasis syndrome. Our study demonstrated that continual exhausting swimming followed by high fat diet could be a method to make a rat model of Qi deficiency and blood stasis syndrome. The rat model could be further utilized in the evaluation of drugs for cardiovascular disease due to its good stability.