浏览全部资源
扫码关注微信
Cloning and expression analysis of Nile tilapia
TNF-R1 andTNF-R2 gene- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 59, Issue 5, Pages: 144-155(2020)
- 作者机构：
中山大学生命科学学院/水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室/ 广东省重要经济鱼类健康养殖工程技术研究中心，广东 广州 510275
- 作者简介：
- 基金信息：
DOI：10.13471/j.cnki.acta.snus.2020.01.09.2020E002
CLC： Q17Published：25 September 2020，
Received：09 January 2020，
扫 描 看 全 文
叶航宇,葛岩岩,吴金英等.尼罗罗非鱼TNF-R1和TNF-R2基因克隆及表达[J].中山大学学报(自然科学版),2020,59(05):144-155.
YE Hangyu,GE Yanyan,WU Jinying,et al.Cloning and expression analysis of Nile tilapia TNF-R1 and TNF-R2 gene[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(05):144-155.
叶航宇,葛岩岩,吴金英等.尼罗罗非鱼TNF-R1和TNF-R2基因克隆及表达[J].中山大学学报(自然科学版),2020,59(05):144-155. DOI： 10.13471/j.cnki.acta.snus.2020.01.09.2020E002.
YE Hangyu,GE Yanyan,WU Jinying,et al.Cloning and expression analysis of Nile tilapia TNF-R1 and TNF-R2 gene[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(05):144-155. DOI： 10.13471/j.cnki.acta.snus.2020.01.09.2020E002.
摘要
为揭示TNF-α受体基因
TNF-R1
和
TNF-R2
在尼罗罗非鱼（
Oreochromis niloticus
）免疫过程的作用，本研究利用RACE技术获得了
TNF-R1
和
TNF-R2
的cDNA。结果表明，
TNF-R1
cDNA全长2 275 bp，ORF区1 335 bp，编码444个氨基酸；
TNF-R2
cDNA全长1 719 bp，ORF区1 476 bp，编码491个氨基酸。TNF-R1含3个富含半胱氨酸结构域（cysteine-rich domain
CRD）和一个死亡结构域（death domain
DD），而TNF-R2有4个CRD，缺少DD。系统进化分析表明：其
TNF-R1
和
TNF-R2
分别与其他脊椎动物
TNF-R1
和
TNF-R2
基因同源性很高，在进化树中
TNF-R1
、
TNF-R2
各自聚类且两者之间分支明显。实时荧光定量PCR结果表明：
TNF-R1
和
TNF-R2
在该鱼体的不同组织中均有表达，其中在鳃、头肾和脾脏中表达水平最高，而在肌肉、垂体中表达量低。用脂多糖（LPS）和多聚肌苷酸-胞苷酸（Poly Ⅰ:C）分别刺激离体孵育的该鱼头肾和脾脏白细胞可不同程度上调
TNF-α
、
TNF-R1
和
TNF-R2
的表达。无乳链球菌（
Streptococcus agalactiae
）感染该鱼可显著上调头肾和脾脏中3种基因的表达。本研究对揭示TNF-R1和TNF-R2免疫调控机制，提高罗非鱼健康养殖水平具有一定参考价值。
Abstract
To reveal the role of TNF-α receptor genes
TNF-R1
and
TNF-R2
in the Nile tilapis (
Oreochromis niloticus
) immune processes
we cloned the full-length cDNA sequences of
TNF-R1
and
TNF-R2
from Nile tilapia by RACE. Sequence analysis showed that the full length of
TNF-R1
cDNA was 2 275 bp
containing an open reading frame of 1 335 bp
and encoding a protein with 444 amino acids; the full length of
TNF-R2
cDNA was 1 719 bp
containing an open reading frame of 1 476 bp
and encoding a protein with 491 amino acids. Protein structure prediction showed that TNF-R1 have three cysteine-rich domains and an intracellular death domain
while TNF-R2 have four cysteine-rich domains and without death domain. Systematic evolutionary analysis showed that the tilapia
TNF-R1
and
TNF-R2
were highly homologous with those of other vertebrates. Phylogenetic tree showed that
TNF-R1
and
TNF-R2
were clustered separately. By qRT-PCR
the highest expression levels of
TNF-R1
and
TNF-R2
mRNA were found in the gill
head kidney and spleen
and the lowest levels were in pituitary and muscle. The expression of
TNF-α
TNF-R1
and
TNF-R2
genes can be increased to varying degrees by stimulating the tilapia kidneys and spleen white cells incubated with LPS and Poly Ⅰ:C
respectively. The expressions of three genes in the head
kidney and spleen can be increased after intra-peritoneal injection of
Streptococcus agalactiae
. This study has some reference value to show the immune regulation mechanisms of
TNF-R1
and
TNF-R2
to improve the level of healthy breeding of tilapia.
关键词
TNF-αTNF-R1TNF-R2罗非鱼免疫无乳链球菌
Keywords
TNF-αTNF-R1TNF-R2Oreochromis niloticusimmunityStreptococcus agalactiae
references
WATT S, TANIA H. TNF/TNFR family members in costimulation of T cell responses[J]. Annual Review of Immunology, 2005, 23(1):23-68.
PETRUS J W N, BOER J A D, LUITEN P G M, et al. Tumor necrosis factor receptor cross-talk[J]. Federation of European Biochemical Societies Journal, 2011, 278(6):888-898.
WEINLICH R, GREEN D R. The two faces of receptor interacting protein kinase-1[J]. Molecular Cell, 2014, 56(4):469-480.
ZHOU X X, TIAN Z Q, WANG Y B, et al. Effect of treatment with probiotics as water additives on tilapia (Oreochromis niloticus) growth performance and immune response[J]. Fish Physiology and Biochemistry, 2010, 36(3):501-509.
WAJANT H, PFIZENMAIER K, SCHEURICH P. Tumor necrosis factor signaling[J]. Cell Death and Differentiation, 2003, 10(1):45-65.
CARPENTIER I, COORNAERT B, BEYAERT R. Function and regulation of tumor necrosis factor receptor type 2[J]. Current Medicinal Chemistry, 2004, 11(16):2205-2212.
DEMPSEY P W, DOYLE S E, HE J Q, et al. The signaling adaptors and pathways activated by TNF superfamily[J]. Cytokine and Growth Factor Reviews, 2003, 14(3/4):193-209.
AGGARWAL B B. Signalling pathways of the TNF superfamily: a double-edged sword[J]. Nature Reviews Immunology, 2003, 3(9):745-756.
GRAYFER L, WALSH J G, BELOSEVIC M. Characterization and functional analysis of goldfish (Carassius auratus L .) tumor necrosis factor-alpha[J]. Developmental and Comparative Immunology, 2008, 32(5):532-543.
HIRONO I, NAM B H, KUROBET, et al. Molecular cloning, characterization, and expression of TNF cDNA and gene from Japanese flounder Paralychthys olivaceus[J]. The Journal of Immunology, 2000, 165(8):4423-4427.
HONG S, LI R, XU Q, et al. Two types of TNF-α exist in teleost fish: phylogeny, expression, and bioactivity analysis of type-II TNF-α3 in rainbow trout Oncorhynchus mykiss[J]. The Journal of Immunology, 2013, 191(12):5959-5972.
卢翠玉, 李炎, 刘庆慧, 等. 免疫刺激诱导草鱼肿瘤坏死因子TNF-α的体外表达特征研究[J]. 水生生物学报, 2016, 40 (3):481-487.
LU C Y, LI Y, LIU Q H, et al. In vitro expression pattern of grass carp tumor necrosis factor (TNF-α) in response to immune stimulation[J]. Acta Hydrobiologica Sinica, 2016, 40 (3):481-487.
PRAVEEN K, EVANS D L, JASOFRIEDMANN L. Constitutive expression of tumor necrosis factor-alpha in cytotoxic cells of teleosts and its role in regulation of cell-mediated cytotoxicity[J]. Molecular Immunology, 2006, 43(3):279-291.
PARK C I, KUROBE T, HIRONO I, et al. Cloning and characterization of cDNAs for two distinct tumor necrosis factor receptor superfamily genes from Japanese flounder Paralichthys olivaceus[J]. Developmental and Comparative Immunology, 2003, 27(5):365-375.
TAKAO S, JACOB C O. Mouse tumor necrosis factor receptor type I: gen omic structure, polymorphism, and identification of regulatory regions[J]. International Immunology, 1993, 5(7):775-782.
GRAYFER L, BELOSEVIC M. Molecular characterization of tumor necrosis factor receptors 1 and 2 of the goldfish (Carassius aurutus L)[J]. Molecular Immunology, 2009, 46(11/12):2190-2199.
RUI W, LI P L, YIN H, et al. Pathogenicity of human ST23 Streptococcus agalactiae to fish and genomic comparison of pathogenic and non-pathogenic isolates[J]. Frontiers in Microbiology, 2017, 8: 1933.
苏友禄, 刘婵, 邓益琴, 等. 罗非鱼无乳链球菌病的研究进展[J]. 大连海洋大学学报, 2019, 34 (5):757-766.
SU Y L, LIU C, DENG Y Q, et al. Research on Streptococcus agalactiae disease in tilapia: a review[J]. Journal of Dalian Fisheries University, 2019, 34 (5):757-766.
HSU S H, TSAI T R, LIN C N, et al. Solamargine purified from Solanum incanum Chinese herb triggers gene expression of human TNFR I which may lead to cell apoptosis[J]. Biochemical and Biophysical Research Communications, 1996, 229(1):1-5.
KIM J W, KWON M G, PARK M A, et al. Molecular identification and expression analysis of a novel tumor necrosis factor receptor from the black rockfish, Sebastes schlegelii[J]. Developmental and Comparative Immunology, 2011, 35(3):258-262.
XING Q, YU Q, DOU H Q, et al. Genome-wide identification, characterization and expression analyses of two TNFRs in Yesso scallop (Patinopecten yessoensis) provide insight into the disparity of responses to bacterial infections and heat stress in bivalves[J].Fish and Shellfish Immunology, 2016, 52:44-56.
DEROUICH-GUERGOUR D, BRENIER-PINCHART M P, AMBROISE-THOMAS P, et al. Tumour necrosis factor alpha receptors: role in the physiopathology of protozoan parasite infections[J]. International Journal for Parasitology, 2001, 31(8):763-769.
WATI S, RAWLINSON S M, IVANOV R A, et al. Tumour necrosis factor alpha (TNF-α) stimulation of cells with established dengue virus type 2 infection induces cell death that is accompanied by a reduced ability of TNF-α to activate nuclear factor κB and reduced sphingosine kinase-1 activity[J]. Journal of General Virology, 2011, 92(4):807-818.
SEGOVIA M, JENKINS J A, PANIAGUACHAVEZ C, et al. Flow cytometric evaluation of antibiotic effects on viability and mitochondrial function of refrigerated spermatozoa of Nile tilapia[J]. Theriogenology, 2000, 53(7):1489-1499.
PRIDGEON J W, KLESIUS P H. Development and efficacy of a novobiocin-resistant Streptococcus iniae as a novel vaccine in Nile tilapia (Oreochromis niloticus)[J]. Vaccine, 2011, 29(35):5986-5993.
PASNIK D J, EVANS J J, KLESIUS P H. Passive immunization of Nile tilapia (Oreochromis niloticus) provides significant protection against Streptococcus agalactiae[J]. Fish and Shellfish Immunology, 2006, 21(4):365-371.
ZHU J J, LI C, AO Q W, et al. Trancriptomic profiling revealed the signatures of acute immune response in tilapia (Oreochromis niloticus) following Streptococcus iniae challenge[J]. Fish and Shellfish Immunololy, 2015, 46(2):346-353.
LAMKANFI M, DIXIT V M. Manipulation of host cell death pathways during microbial infections[J]. Cell Host and Microbe, 2010, 8(1):44-54.
0
Views
1
下载量
0
CSCD
- L-PDFDownload
- BibTeXDownload
- EndnoteDownload
- RefMan Download
- NoteExpressDownload
- NoteFirstDownload
Publicity Resources
Related Articles
Related Author
Related Institution
- Postal code：510275
- Tel：020-84112585，84113223 Email：xuebaozr@mail.sysu.edu.cn
- Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19
京公网安备11010802024621 - It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
- Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.
