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Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (
Scortum barcoo )- Acta Scientiarum Naturalium Universitatis Sunyatseni Vol. 59, Issue 4, Pages: 7-18(2020)
- 作者机构：
中山大学生命科学学院/水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室/ 广东省重要经济鱼类健康养殖工程技术研究中心，广东 广州 510275
- 作者简介：
- 基金信息：
DOI：10.13471/j.cnki.acta.snus.2019.10.18.2019E026
CLC： Q17Published：20 July 2020，
Received：29 October 2019，
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高凇泽,孙彩云,吴金英等.澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达[J].中山大学学报(自然科学版),2020,59(04):7-18.
GAO Songze,SUN Caiyun,WU Jinying,et al.Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(04):7-18.
高凇泽,孙彩云,吴金英等.澳洲宝石鲈热休克蛋白90-β基因（HSP90-β）的cDNA克隆与表达[J].中山大学学报(自然科学版),2020,59(04):7-18. DOI： 10.13471/j.cnki.acta.snus.2019.10.18.2019E026.
GAO Songze,SUN Caiyun,WU Jinying,et al.Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)[J].Acta Scientiarum Naturalium Universitatis Sunyatseni,2020,59(04):7-18. DOI： 10.13471/j.cnki.acta.snus.2019.10.18.2019E026.
摘要
热休克蛋白（heat shock proteins，HSPs）在鱼类的应激与免疫反应中起着重要的作用，HSP90-β是该家族的重要成员。为了探讨澳洲宝石鲈（
Scortum barcoo
）HSP90-β在免疫应答中的作用，本研究首次克隆得到了澳洲宝石鲈
HSP90-β
的全长cDNA序列。该序列长2 708 bp，其中5' UTR长86 bp，3' UTR长444 bp，ORF长2 178 bp，编码725个氨基酸，相对分子质量约为83 200，具有5个HSP90家族的特征序列，没有信号肽序列和跨膜结构域，与其他鱼类HSP90-β氨基酸的序列一致性皆超过90%。正常情况下，澳洲宝石鲈
HSP90-β
mRNA主要在肝脏表达。而感染无乳链球菌(
Streptococcus agalactiae
)后，其在肝组织中的表达呈先降低后增加的趋势，在脾脏、头肾和脑组织则呈先上升后下降的趋势。这些结果表明澳洲宝石鲈HSP90-β可能参与了无乳链球菌感染引发的免疫反应。
Abstract
Heat shock proteins (HSPs) play important roles in fish stress and immune response
and HSP90-β is an important member of the HSPs family. In the present study
to investigate the possible role of HSP90-β in the immune response
the full-length cDNA sequence of
HSP90-β
was first cloned from jade perch (
Scortum barcoo
). The size of the full-length cDNA of jade perch
HSP90-β
is 2 708 bp
of which 5' UTR is 86 bp
3' UTR is 444 bp
and ORF is 2 178 bp
which encodes 725 amino acids. The molecular weight of jade perch HSP90-β is predicted as 83 200. And this protein owns five characteristic sequences of the HSP90 family. Moreover
it has no signal peptide and transmembrane domain. Phylogenetic analysis showed that the amino acid sequence identity of jade perch HSP90-β is more than 90% with those of other fishes. Under normal circumstances
the
HSP90-β
mRNA of jade perch is mainly expressed in the liver. After infection with
Streptococcus agalactiae
the
HSP90-β
mRNA expression decreased first and then increased in liver. Differently
it increased first and then decreased in spleen
head kidney and brain. This result suggests that the HSP90-β may be involved in the immune response elicited by
S. agalactiae
infection in jade perch.
关键词
澳洲宝石鲈HSP90-β分子克隆表达
Keywords
Scortum barcooHSP90-βmolecular cloningexpression analysis
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