A novel approach to prepare the difference samples of traditional Chinese herbal formulae (CHF) was established，the strategy of the approach are: regarding the herb as the test factors

defining the suitable test levels based on the original herb proportion

designing the different herb proportion using Uniform Design method

preparing the different samples according to standard process

analyzing the HPLC chromatography of the difference samples using Cluster Analysis. Different from traditional pattern of separating and combining the component one by one

the proposed approach re-adjusts the herbs as a whole with the advantages of low difficulty and high applicability

conforming to the CHF characters of mixture

integrity

and unity.