FT-IR and FT-Raman are applied to characterize the molecular vibrational spectra of Clobetasol Propionate. The second derivative spectra are used to enhance the spectra‘s resolution. The results show that the spectra’s bands of Clobetasol Propionate can be more sufficiently assigned by combining two kinds molecular spectra. The research proves that the structure information of medicine can be obtained under no-destructive state.