Rhizopus oryzae lipase preparation and its application in the conversion of waste cooking oil

JIANG Sujun; LIN Jianghai; LV Xiaojing; XIAO Wenjuan; GONG Yingxue; LIU Zehuan

doi:10.13471/j.cnki.acta.snus.2016.02.020

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Rhizopus oryzae lipase preparation and its application in the conversion of waste cooking oil

更新时间：2023-12-11

- Rhizopus oryzae lipase preparation and its application in the conversion of waste cooking oil
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 55, Issue 2, Pages: 111-116(2016)
- 作者机构：
  
  暨南大学生命科学技术学院,广东,广州,510632
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2016.02.020
  CLC：
- Published：2016，
  
  Published Online：25 March 2016，
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JIANG Sujun, LIN Jianghai, LV Xiaojing, et al. Rhizopus oryzae lipase preparation and its application in the conversion of waste cooking oil. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 55(2):111-116(2016)
DOI：

JIANG Sujun, LIN Jianghai, LV Xiaojing, et al. Rhizopus oryzae lipase preparation and its application in the conversion of waste cooking oil. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 55(2):111-116(2016) DOI： 10.13471/j.cnki.acta.snus.2016.02.020.

摘要

利用脂肪酶把餐厨废弃油脂转化成为生物柴油能够达到绿色化、资源化处理餐厨垃圾的目的。从米根霉CICC3005 cDNA文库扩增得到脂肪酶基因（proROL），并克隆到毕赤酵母组成型表达载体pGAPZαA中，电转入毕赤酵母X-33中构建重组毕赤酵母，SDS-PAGE电泳发酵液上清，结果显示重组酶的相对分子质量约为35 000。以橄榄油为底物测得脂肪酶活性为(426.6±08) U/mL。利用重组的米根霉脂肪酶对餐厨废弃油脂进行转酯化反应，以乙醇为酰基受体制备脂肪酸乙酯，在水含量为5%，醇油摩尔比为4∶1，酶添加量为10%的条件下得到脂肪酸乙酯的最高得率为49%。

Abstract

Using lipase to change waste cooking oil into biodiesel can achieve the goal of green

recycling of food waste. A gene encoding Rhizopus oryzae lipase containing prosequence(ProROL) was cloned into the pGAPZαA and electrotransformed into the Pichia pastoris X-33 strain. The lipase was functionally expressed and secreted with a molecular weight of 35 000. The lipase activity was (426.6±0.8) U/mL when using olive oil as substract. Using waste cooking oil as the raw material and alcohol as acyl receptor

lipase as catalyst for the production of fatty acid ethyl ester

49% of the fatty acid ethyl ester yield was obtained when the water content is 5%

the alcohol oil molar ratio is 4∶1

and lipase adding amount is 10%

under this condition the fatty acid ethyl ester yield is 49%.

关键词

餐厨废弃物生物柴油米根霉脂肪酶毕赤酵母

Keywords

food wastebiodieselRhizopus oryzaelipasePichia pastoris

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