MSAP analysis of Lycoris longituba organogenesis in tissue culture

LIU Hexia; LI Bo; ZHOU Jian

doi:10.13471/j.cnki.acta.snus.2016.02.019

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MSAP analysis of Lycoris longituba organogenesis in tissue culture

更新时间：2023-12-11

- MSAP analysis of Lycoris longituba organogenesis in tissue culture
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 55, Issue 2, Pages: 102-110(2016)
- 作者机构：
  
  1. 广西壮族自治区中国科学院广西植物研究所园林园艺研究中心,广西,桂林,541006
  2. 
  3. 桂林师范高等专科学校化学与药学系,广西,桂林,541001
- 作者简介：
- 基金信息：
- DOI：10.13471/j.cnki.acta.snus.2016.02.019
  CLC：
- Published：2016，
  
  Published Online：25 March 2016，
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LIU Hexia, LI Bo, ZHOU Jian. MSAP analysis of Lycoris longituba organogenesis in tissue culture. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 55(2):102-110(2016)
DOI：

LIU Hexia, LI Bo, ZHOU Jian. MSAP analysis of Lycoris longituba organogenesis in tissue culture. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 55(2):102-110(2016) DOI： 10.13471/j.cnki.acta.snus.2016.02.019.

摘要

采用甲基化敏感扩增多态性技术，从 64 对 MSAP 选扩引物中，选出扩增清晰、可辨且可重复的 51 对 MSAP 引物组合，扩增获得长筒石蒜种子胚、愈伤组织、苗等3个不同发育时期的 DNA胞嘧啶甲基化修饰水平及 MSAP扩增图谱。结果表明，种子胚基因组中有 68.0%（全甲基化 50.2%、半甲基化 49.8%）、愈伤组织60.0%（全甲基化 52.2%、半甲基化 47.8%）、苗64.3%（全甲基化 40.8%、半甲基化 59.2% ）的CCGG/GGCC 位点发生胞嘧啶甲基化。3个不同时期甲基化的水平互有差异，依据超甲基化/去甲基化的修饰机理分析，甲基化水平的变化或许导致了长筒石蒜不同的器官发生。

Abstract

In this study

DNA cytosine methylation modification level and MSAP amplification graphs of Lycoris longituba were obtained from seed embryo

callus and seedling via methylation sensitive amplification polymorphism technology. We found that 51 pairs of MSAP selective amplifying primer combinations were good in 64 pairs of the MSAP selective amplifying primer combinations.The results showed that the cytosine methylation rates were 68.0% in seed embryo (full methylation:50.2% and half methylation:49.8%)

60.0% in callus(full methylation:52.2% and half methylation:47.8%) and 64.3% in seeding(full methylation:40.8% and half methylation:59.2%).The level of methylation differed with each other in the three different statges. So

it is suggested that changes of the level of methylation may had led to their different organs of Lycoris longituba based on the modification mechanism of super methylation/demethylation analysis.

关键词

长筒石蒜MSAP器官发生

Keywords

Lycoris longitubaMSAPorganogenesis

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State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yatsen University, Guangzhou

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