To study the secondary metabolites of endophytic fungus Neurospora sp. DHLRH-F from Meconopsis grandis Prain from Tibet

its fermentation products were isolated and purified by silica gel

Sephadex LH-20

ODS-18 column chromatography

and HPLC. The structures of obtained compounds were identified by NMR and MS analysis

and the absorption spectrums of the compounds-iron (Ⅱ) or iron (Ⅲ) were investigated by UV/Vis spectroscopy

respectively. The results showed that three polyphenols were isolated from the fermentation compounds of Neurospora sp. DHLRH-F. Their structures were identified as 2-chloro-6-methoxybenzene-1

4-diol (1)

6-epi-5′-hydroxymycosporulone (2)

and mycophenolic acid (3); compound 1 was a new natural product and showed a certain complexation ability for iron (Ⅲ). We firstly reported about secondary metabolites of endophytic fungus from Meconopsis Vig..