Cloning and Characterization Analysis of Heat Shock Protein 90 Gene from Harmonia axyridis( Pallas)

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Cloning and Characterization Analysis of Heat Shock Protein 90 Gene from Harmonia axyridis( Pallas)

更新时间：2023-12-11

- Cloning and Characterization Analysis of Heat Shock Protein 90 Gene from Harmonia axyridis( Pallas)
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 49, Issue 2, Pages: 72-78(2010)
- 作者机构：
  
  1. 2北京市农林科学院植保环保所,北京,100089
  2. 3中山大学生命科学学院,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010，
  
  Published Online：25 March 2010，
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Cloning and Characterization Analysis of Heat Shock Protein 90 Gene from Harmonia axyridis( Pallas). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 49(2):72-78(2010)
DOI：

Cloning and Characterization Analysis of Heat Shock Protein 90 Gene from Harmonia axyridis( Pallas). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 49(2):72-78(2010) DOI：

摘要

采用同源克隆和锚定PCR技术

从异色瓢虫 Harmonia axyridis (Pallas)中克隆到热休克蛋白HSP 90 基因的cDNA全序列（Genbank number: FJ501962）。cDNA全长2 480 bp，包含3′非编码区域(UTR)为200 bp和5′UTR为126 bp，开放阅读框(ORF) 长2.154 bp，编码717个氨基酸。预测的相对分子质量为82.230

理论等电点为4.96，无糖基化位点、跨膜结构和信号肽。在N端具有 HSP 90 基因保守的ATPase结构，含有HSP90家族的C末端的保守序列EEVD。与赤拟谷盗 Tribolium castaneum 相比较，同源性高达90%，系统发育分析也表明两者的亲缘关系最近。 HaaHSP 90 基因的克隆和比较分析为进一步深入研究异色瓢虫的抗逆机理及其进化具有重要意义。

Abstract

The heat shock protein 90 (HSP 90) gene from Harmonia axyridis (Pallas) was cloned using techniques of homological cloning and anchored PCR. The full length of cDNA sequence is 2 480 bp（Genbank number: FJ501962）

containing a 3′UTR (untranslated region) of 200 bp

a 5′UTR of 126 bp

and an ORF of 2 154 bp which encodes a polypeptide of 717 amino acids with an estimated molecular weight of 82 230 and p I of 4.96. There are no glycosylation sites

putative transmembrane domain and signature peptide. It contains ATPase conservative domain of (HSP90) in Nsite and EEVD signature sequence on Csite. Compared with Tribolium castaneum

they are 90% identities and Phylogenetic analysis shows that these two species are most closely related. Cloning and comparison analysis of HSP 90 genes from Harmonia axyridis (Pallas) could be useful in the studies of antistress mechanism

tolerance improvement breeding and evolution for these animals.

关键词

异色瓢虫HSP 90 克隆特性分析序列分析

Keywords

Harmonia axyridisHSP 90cloningcharacterization analysissequence analysis

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Related Institution

广东教育学院生物系

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School of Life Science, Zhongshan University

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