The Effects of Benzo［a］Pyrene(BaP) Exposure on the CYP1A1 mRNA and AhR2 mRNA Expression of Red Seabream (Pagrus major)

您当前的位置：

首页 >

文章列表页 >

The Effects of Benzo［a］Pyrene(BaP) Exposure on the CYP1A1 mRNA and AhR2 mRNA Expression of Red Seabream (Pagrus major)

更新时间：2023-12-11

- The Effects of Benzo［a］Pyrene(BaP) Exposure on the CYP1A1 mRNA and AhR2 mRNA Expression of Red Seabream (Pagrus major)
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 49, Issue 3, Pages: 93-97(2010)
- 作者机构：
  
  1. 近海海洋环境科学国家重点实验室//厦门大学环境科学研究中心,福建,厦门,361005
  2. 
  3. 泉州师范学院生物系,福建,泉州,362000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010，
  
  Published Online：25 May 2010，
扫描看全文
The Effects of Benzo［a］Pyrene(BaP) Exposure on the CYP1A1 mRNA and AhR2 mRNA Expression of Red Seabream (Pagrus major). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 49(3):93-97(2010)
DOI：

The Effects of Benzo［a］Pyrene(BaP) Exposure on the CYP1A1 mRNA and AhR2 mRNA Expression of Red Seabream (Pagrus major). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 49(3):93-97(2010) DOI：

摘要

通过水体暴露方式对海水养殖真鲷进行BaP持续染毒，利用实时定量PCR技术研究了真鲷细胞色素P450基因(CYP1A1)和芳香烃受体基因(AhR2)随BaP暴露剂量、时间的动力学变化。结果发现，01～15 μg/L环境浓度的BaP能够显著性诱导CYP1A1基因和AhR2基因的表达，且AhR2 mRNA早于CYP1A1 mRNA被诱导表达；BaP持续暴露48 h，CYP1A1和AhR2基因的表达水平均随暴露时间的延长而显著升高，染毒72 h后又回复到本底水平，实验表明这两个基因的表达与BaP的暴露剂量和暴露时间之间具有显著性的剂量-效应和时间-效应关系。

Abstract

The gene expression patterns of CYP1A1 and Aryl hydrocarbon receptor (AhR2) of red seabream (〖WTBX〗Pagrus major〖WTBZ〗) were both measured using realtime quantitative PCR (qPCR) when fish exposed to environmentally relevant concentration of BaP (01

05 and 10 μg/L

respectively). The results showed that CYP1A1 mRNA and AhR2 mRNA could be induced significantly

besides

the time of AhR2 mRNA induced ahead of the time of CYP1A mRNA induced. The two genes were induced markedly at the begining of BaP exposure

and then decreased to the basal levels after 72 h. The results demonstrate that BaP can regulate CYP1A1 and AhR2 transcript in a dose and time dependent manner.

关键词

苯并［a］芘(BaP)真鲷实时定量PCR细胞色素P450(CYP1A1)芳香烃受体(AhR)

Keywords

Benzo［a］Pyrene (BaP)Pagrus majorrealtime quantitative PCRcytochromeP450 (CYP1A1)aryl hydrocarbon receptor (AhR)

references

Views

152

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Detection of Genetically Modified Soybean by TaqMan MGB Realtime PCR

Related Author

Huang Dongdong

WENG Shaoping

LV Ling

CHANG Di

HE Jianguo

Related Institution

School of Life Sciences,Sun Yatsen University,Guangzhou 510275, China)

Postal code：510275
Tel：020-84112585，84113223 Email：xuebaozr@mail.sysu.edu.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰