Taxonomy Study by Fingerprint of POD and PPO Isozymes in Brassica Vegetables

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Taxonomy Study by Fingerprint of POD and PPO Isozymes in Brassica Vegetables

更新时间：2023-12-11

- Taxonomy Study by Fingerprint of POD and PPO Isozymes in Brassica Vegetables
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 49, Issue 4, Pages: 106-110(2010)
- 作者机构：
  
  佛山科学技术学院园艺系,广东,佛山,528231
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010，
  
  Published Online：25 July 2010，
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Taxonomy Study by Fingerprint of POD and PPO Isozymes in Brassica Vegetables. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 49(4):106-110(2010)
DOI：

Taxonomy Study by Fingerprint of POD and PPO Isozymes in Brassica Vegetables. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 49(4):106-110(2010) DOI：

摘要

采用垂直平板聚丙烯酰胺凝胶电泳技术对芸薹属21个品种根POD同工酶和PPO同工酶进行了研究，并用类平均法(UPGMA)进行了聚类分析。结果表明：大白菜、小白菜、菜心、油菜、壬生菜、京水菜、芜菁属于芸薹种下的亚种或变种，结球甘蓝、紫甘蓝、抱子甘蓝、羽衣甘蓝、花椰菜、青花菜、紫花菜、苤蓝、芥蓝属于甘蓝种下的亚种或变种，雪里蕻、结球芥菜属于芥菜种下的变种。从蛋白质分子水平上分析了芸薹属三大类蔬菜的亲缘关系，并确定了特征指纹图谱。

Abstract

Peroxidase (POD) isozyme and polyphenol oxidase (PPO) isozyme in the roots of 21 Brassica cultivars were studied by the technology of vertical slab polyacrylamide gel electrophoresis

and cluster analysis was made using UPGMA method. The results showed that Chinese cabbage

pakchoi

flowering Chinese cabbage and turnip were classified into subspecies or varieties of Brassica campestris L. Cabbage

purple cabbage

brussels sprouts

kale

cauliflower

broccoli

purple cauliflower

kohlrabi and Chinese kale were classified into subspecies or varieties of B.oleracea L. Leaf mustard and head mustard were classified into subspecies or varieties of B.juncea Coss. The genetic relationship of 3 groups of Brassica vegetables was analyzed at the protein molecule level and the isozyme fingerprint identifying them was determined.

关键词

芸薹属同工酶指纹图谱聚类分析

Keywords

Brassicaisozymefingerprintcluster analysis

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Related Institution

Institute of Physiology and Ecology of Medicinal Plants， International Institute of Translational Medicine of Traditional Chinese Medicine， School of Chinese Materia Medica， Guangzhou University of Chinese Medicine

中山大学生物学系

Department of Biology,Zhongshan University

华中农业大学水产学院

Research Centre of Biotechnology, Zhongshan University

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