Singapore grouper iridovirus (SGIV) is a major pathogen resulting in heavy economic losses to grouper aquaculture. In this study

recombant eukaryotic vector pEGFPICP18 which inserted with SGIV ICP18 gene was transfected into Fathead minnow (FHM) cells

and ICP18GFP fusion protein was successfully expressed in FHM cells with a finely punctate cytoplasmic pattern. Candidate siRNA targeting SGIV ICP18 gene (siRNAICP18) was designed and chemically synthesized．To investigate the inhibition effect of siRNAICP18

pEGFPICP18 and siRNA were cotransfected into FHM cells

and the green fluorescence was observed by fluorescence microscope after transfection. The green fluorescence in FHM cells cotransfected with pEGFPICP18 and siRNAICP18 were 60%~80% fewer than that of negative control

which show the siRNAICP18 can effectively silence the extrinsic SGIV ICP18 gene in FHM cells during 24~48 h after transfection.