The dissociation and culture in different artificial cerebrum spinal fluids (ACSF) of facial lobe slice isolated from Southern catfish (

Silurus meridionalis

Chen) are described. To observe the physiological functions in information coding of primary gustatory central neurons and to elaborate the mechanism of information identification in fish

we established the teleost infrared visual patch clamp method. According to experimental requirement

low calcium ACSF contained HEPES

Vc

TU and kynurenic acid could be selected for normal brain slices culture at the temperature of 25 ℃within 2 h. The morphology and electrophysiological characteristics of neurons were observed noninvasively by brain slice patch clamp with infrared videomicroscopy. There were two types of neurons (pyriform and oval neurons) in facial lobe. They all had no or low rate of spontaneous discharges and frequency adaptation. Pyriform neuron had lower membrane resistance but with higher evoked discharge frequency.