Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides)

PING Hailin; WU Jinying; XU Shengwei; HU Kaishun; DUAN Zhigang

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Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides)

更新时间：2023-12-11

- Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides)
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 50, Issue 1, Pages: 92-98(2011)
- 作者机构：
  
  中山大学生命科学学院水生经济动物研究所∥广东省水生经济动物良种繁育重点实验室,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011，
  
  Published Online：25 January 2011，
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PING Hailin, WU Jinying, XU Shengwei, et al. Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 50(1):92-98(2011)
DOI：

PING Hailin, WU Jinying, XU Shengwei, et al. Molecular Cloning and Expression Analysis of Transforming Growth Factorβ1(TGFβ1) from OrangeSpotted Grouper (Epinephelus coioides). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 50(1):92-98(2011) DOI：

摘要

转化生长因子β1（TGFβ1）是一种高度保守的多效细胞因子。通过分子克隆得到了斜带石斑鱼Epinephelus coioidesTGFβ1 cDNA全长序列，全长为2 477 bp，开放阅读框为1 161 bp，编码一种含386个氨基酸的多肽。Realtime PCR分析表明，TGFβ1基因在健康斜带石斑鱼所取组织中均有表达，肾脏中表达最高，其次是头肾和脾脏。用PolyI:C或ConA刺激斜带石斑鱼头肾淋巴细胞，TGFβ1表达量明显上升，且在刺激4 h后达到最高值。用PolyI:C刺激16、24 h后，TGFβ1几乎不表达，而用ConA刺激实验组，TGFβ1表达量一直高于对照组。结果说明，斜带石斑鱼TGFβ1基因结构和表达特征与其他硬骨鱼类具有高度相似性。

Abstract

Transforming growth factorβ1 (TGFβ1) is a highly conserved pleiotropic cytokine. In this study

we presented the bioinformatics analysis of TGFβ1 fulllength cDNA sequences from orangespotted grouper (Epinephelus coioides). The full length cDNA is 2477 bp

which contains an ORF of 1161 bp

encoding a 386 amino acids protein. Realtime PCR results showed that TGFβ1 mRNA was predominantly expressed in the kidney

followed by head kidney and spleen of the healthy orangespotted grouper. The expression of TGFβ1 in isolated headkidney lymphocytes was upregulated treated with PolyI:C or ConA

and peaked at 4 hours after stimulation. Lymphocytes groups which were treated with PolyI:C had little TGFβ1 expression after 16 and 24 hours

while the expression of TGFβ1 was significantly higher than the control groups during the whole experimental processing time treated with ConA. In conclusion

we found the structure and expression pattern of grouper TGFβ1 was similar to those of other fish.

关键词

TGFβ1斜带石斑鱼Epinephelus coioides克隆表达分析

Keywords

TGFβ1Epinephelus coioidescloningexpression

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