hSSB1 is a key signaling moleculue that participates in DNA damage response. In this study

the cDNA of

hSSB1

gene amplified by RT-PCR was inserted into the retroviral vector pBABE. The recombinant positive plasmid clone was identified by endonuclease digestion

PCR amplification and sequencing analysis. The retroviral expression vector pBABE-

hSSB1

and PIK packaging plasmid were cotransfected into 293T cells to produce the retrovirus. The packaging retrovirus was then infected into cancer cells and the overexpression cells were selected with puromycin. pBABE-

hSSB1

positive clones have been validated to be correct by restriction endonuclease

PCR amplification and DNA sequencing analysis. The protein level of hSSB1 in pBABE-

hSSB1

transfected cancer cell line was significantly up-regulated as validated by Western blotting. Our data indicate that the recombinant plasmid of pBABE-

hSSB1

was successfully constructed and transfected stably into cancer cells. It established a favorable foundation for further functional study.