This study investigated the transformation of pleiotropic gene

AtCPR5

into Zhonghua 11(

Oryza sativa

L. subsp.

japonica

) mediated by

Agrobacterium tumefaciens

and optimized the regeneration system and genetic transformation system. It was showed the induction rate of callus could be increased to 100 % and the period of induction could be shortened under the condition of continuous light and 32 ℃ compared with the condition of dark and 27 ℃. After predifferentiation

the differentiation rate and regeneration rate could be raised to 100 % and 90.50 %

respectively

by adding 5 mg/l 6BA and 1 mg/l NAA in the differential medium. Additionally

the higher transformation efficiency was obtained with the infected concentration of

A.tumefaciens

to an

A

600 of 0.05～0.1

5 minutes of infection and 3 days of cocultivation by placing a filter moistened with AAI medium. Furthermore

the results of PCR

Southern blot and semi-quantitative RTPCR indicated

AtCPR5

gene has been integrated into rice genome with single copy

however

the expression level 5 are different in transgenic plantlets.