Determination of the Contents of Geniposide and Crocin in Fructus gardeniae  by Capillary Electrophoresis

HUANG Baomei; YAO Chengwei; MO Jinyuan; BIAN Qingquan; WANG Zhiguo

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Determination of the Contents of Geniposide and Crocin in Fructus gardeniae by Capillary Electrophoresis

更新时间：2023-12-11

- Determination of the Contents of Geniposide and Crocin in Fructus gardeniae by Capillary Electrophoresis
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 50, Issue 5, Pages: 83-86(2011)
- 作者机构：
  
  1. 绵阳师范学院化学系,四川,绵阳,621000
  2. 2中国空气动力研究与发展中心设备设计及测试技术研究所,四川,绵阳,622653
  3. 3中山大学化学与化学工程学院,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011，
  
  Published Online：25 September 2011，
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HUANG Baomei, YAO Chengwei, MO Jinyuan, et al. Determination of the Contents of Geniposide and Crocin in Fructus gardeniae by Capillary Electrophoresis. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 50(5):83-86(2011)
DOI：

HUANG Baomei, YAO Chengwei, MO Jinyuan, et al. Determination of the Contents of Geniposide and Crocin in Fructus gardeniae by Capillary Electrophoresis. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 50(5):83-86(2011) DOI：

摘要

采用毛细管电泳安培法测定了栀子

Fructus gardeniae

中京尼平甘和藏红花素的含量。在20 mmol/L Tris-HCl 缓冲溶液(pH = 8.50)， 25 kV高压，0.86 V工作电极电位的条件下，在4 min内实现了两种被测组分的有效分离。测得四川栀子中京尼平甘和藏红花素的含量为32.46 mg/g和8.527 mg/g，浙江栀子中京尼平甘和藏红花素的含量为26.51 mg/g和7.028 mg/g。浙江栀子中京尼平甘和藏红花素的平均回收率分别为99.64% 和100.2%，四川栀子中京尼平苷和藏红花素的平均回收率分别为99.73% 和99.92%。该法快速准确，为栀子中京尼平甘和藏红花素的质量控制提供了新方法。

Abstract

The capillary electrophoresis was used for the determination of the content of Geniposide and Crocin in

Fructus gardeniae

. The effective separated components were achieved by using separation voltage 25 kV

injection time 8 seconds and detection potential at 0.86 V in 20 mmol/L TrisHCl buffer condition (pH=8.50). The contents of Geniposide and Crocin in Sichuan

Fructus gardeniae

were determined to be 32.46 mg/g and 8.527 mg/g. The contents of Geniposide and Crocin in Zhejiang

Fructus gardeniae

were determined to be 26.51 mg/g and 7.028 mg/g. The average recovery ratios of Geniposide and Crocin in Sichuan

Fructus gardeniae

were 99.73% and 99.92%. The average recovery ratios of Geniposide and Crocin in Zhejiang

Fructus gardeniae

were 99.64 % and 100.2%. A novel method of the quality control of Geniposide and Crocin in

Fructus gardeniae

was built up.

关键词

毛细管电泳栀子Fructus gardeniae京尼平甘藏红花素

Keywords

capillary electrophoresisFructus gardeniaeGeniposideCrocin

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Related Institution

Chemical Department of Mianyang Normal University

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Facility Design and Instrumentation Institute of CARDC,Mianyang 6226533

School of Chemistry and Chemical Engineering,Sun Yatsen University

．中山火炬职业技术学院生物医药系

Postal code：510275
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