Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.)

SU Shengyan; DONG Zaijie; ; YUAN Xinhua; LIANG Zhengyuan; QU Jiangqi; ZHANG Jianqiao; LIU Wei; MA Liangxiao; XU Pao

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Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.)

更新时间：2023-12-11

- Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.)
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 51, Issue 2, Pages: 77-85(2012)
- 作者机构：
  
  1. 南京农业大学无锡渔业学院,江苏,无锡,214081
  2. 
  3. 中国水产科学研究院淡水渔业研究中心∥农业部淡水渔业和种质资源利用重点实验室，江苏,无锡,214081
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012，
  
  Published Online：25 March 2012，
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SU Shengyan, DONG Zaijie, , et al. Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 51(2):77-85(2012)
DOI：

SU Shengyan, DONG Zaijie, , et al. Clone of IGF2b Introns, Analysis of Its Genomic Sequence and Construction of Lentiviral Vector(Lenti-IGF2-IRES-EGF) in Common Carp (Cyprinus carpio L.). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 51(2):77-85(2012) DOI：

摘要

为了了解鲤

IGF2b

IGF2

IGF2b

IGF2b-

IGF2b

Abstract

To explore the relationship between the

IGF2b

gene and growth traits

we cloned the introns of

IGF2b

in common carp (Cyprinus carpio ) muscle and analysis its characters. Further more

the lentiviral vector(Lenti-

IGF2-

IRES-EGF) was constructed and its activity was also researched. The results showed that: the length of 5 173 bp DNA sequence of 

IGF2b

(HM755899) was get

including 3 introns and 4 exons. Two CpG islands in the 3′untranslated region and (T)n repeat sequence in the 5′untranslated region were found. Besides these

the lentiviral vector(Lenti-

IGF2b-

IRES-EGF) was constructed successfully

which was cotransfected into 293T cells with the GFP expression. The higher expression of IGF2 was found in 293T cells by Real time PCR. These illustrated that these study may be lay on solid foundation for relationship between

IGF2b

genetic diversity

expression and common carp growth traits.

关键词

鲤IGF2b内含子慢病毒载体

Keywords

common carpIGF2bintronslentiviral vector

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