Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO 14)Under Various Abiotic Stresses

LIU Juhua; DENG Chengju; JIN Zhiqiang; XIE Xueli; JIA Caihong; ZHANG Jianbin; XU Biyu

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Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO 14)Under Various Abiotic Stresses

更新时间：2023-12-11

- Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO 14)Under Various Abiotic Stresses
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 50, Issue 5, Pages: 87-92(2011)
- 作者机构：
  
  1. 中国热带农业科学院热带生物技术研究所∥农业部热带生物技术重点开放实验室,海南,海口,571101
  2. 中国热带农业科学院海口试验站∥香蕉研究所,海南,海口,570102
  3. 
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2011，
  
  Published Online：25 September 2011，
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LIU Juhua, DENG Chengju, JIN Zhiqiang, et al. Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO 14)Under Various Abiotic Stresses. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 50(5):87-92(2011)
DOI：

LIU Juhua, DENG Chengju, JIN Zhiqiang, et al. Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO 14)Under Various Abiotic Stresses. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 50(5):87-92(2011) DOI：

摘要

为研究香蕉乙二醛酶基因的功能，根据香蕉果实采后早期成熟的抑制缩减杂交文库（suppression subtractive hybridization library，SSH）获得的香蕉乙二醛酶基因片段，首次从香蕉中克隆了乙二醛酶基因的cDNA全长

命名为 MaGLO 14 。该cDNA的ORF全长1 074 bp，编码358个氨基酸。BlastX分析表明，该基因cDNA推导的氨基酸序列与云杉（ABK22263）、葡萄（CBI23235）、葡萄柚（CAB09799）、棉花（ACJ11750）和蓖麻（EEF43857）有较高的一致性，分别为82%、83%、82%、80%、82%。组织特异性表达结果显示，该基因在香蕉根、茎、叶、花、果实中均有表达。在NaCl、低温、乙烯利胁迫处理后基因呈现上调表达；在干旱、涝害胁迫处理后基因呈现下调表达。该基因转化烟草显示能够增强转基因烟草离体叶片耐盐性。

Abstract

In order to identify the function of glyoxalase gene in banana

a full length cDNA of glyoxalase gene named MaGLO 14 had been isolated based on the partial sequence cloned by SSH previously from postharvest banana fruit. MaGLO 14 contained a 1 074 bp ORF and encoded 358 amino acids. NCBI Blastx results showed that the deduced amino acid of MaGLO 14 shared identities of 82%

83%

82%

80%

82% with Picea sitchensis(ABK22263)

Vitis vinifera (CBI23235)

Citrus paradisi (CAB09799)

Gossypium hirsutum (ACJ11750)

and 〖WTBX〗Ricinus communis(EEF43857)

respectively. Tissuespecific expression results indicated that MaGLO 14 constitutively expressed in banana root

stem

leaf

flower and fruit. The expression levels were upregulated under NaCl

chilling and ethepon treatments. The expression levels were downregulated under drought and waterlogging treatments. And the MaGLO 14 transgenic tobacco leaves showed enhanced salt-tolerance.

关键词

香蕉乙二醛酶克隆表达分析胁迫转基因

Keywords

bananaglyoxalasecloneexpression analysisstressgene transformation

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