A Preliminary Study on the Tetracycline Resistance Genes in the Livestock Soil, South China

ZOU Shichun; LI Qing; HE Zhumei

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A Preliminary Study on the Tetracycline Resistance Genes in the Livestock Soil, South China

更新时间：2023-12-11

- A Preliminary Study on the Tetracycline Resistance Genes in the Livestock Soil, South China
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 51, Issue 6, (2012)
- 作者机构：
  
  1. 中山大学海洋学院水产品安全教育部重点实验室,广东,广州,510275
  2. 中山大学生命科学学院水产品安全教育部重点实验室,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012，
  
  Published Online：25 November 2012，
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ZOU Shichun, LI Qing, HE Zhumei. A Preliminary Study on the Tetracycline Resistance Genes in the Livestock Soil, South China. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 51(6).(2012)
DOI：

ZOU Shichun, LI Qing, HE Zhumei. A Preliminary Study on the Tetracycline Resistance Genes in the Livestock Soil, South China. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 51(6).(2012) DOI：

摘要

抗生素作为禽畜疾病预防及治疗药物、生长促进剂、饲料添加剂等被广泛应用于养殖业并进入环境，造成了环境中抗性耐药菌和抗性基因（Antibiotic Resistance Genes，ARGs）的日益增加，从而影响人体健康和生态环境。因此，ARGs作为一种新型环境污染物已经引起了人们的广泛关注。本工作以珠海某些养殖场及周边土壤中的微生物及其ARGs为主要研究对象，采用聚合酶链反应（PCR）研究了养殖场土壤细菌DNA中9种四环素抗性基因存在和污染水平。结果表明，所研究的9种四环素抗性基因中除tetD之外，其他8种抗性基因（tetA、tetB、tetC、tetG、tetL、tetO、tetQ和tetX）均可检出，其中tetG的样本检出率达到94%；另外，对其中常见的4种ARGs（tetA、tetC、tetG和tetX）的荧光实时定量PCR定量结果显示，在这4种基因中，以tetC在样品中的丰度最高，tetG次之。在16个土壤采样点中发现，tetC和tetG的copies/g DNA达到10

量级均占50%，其他基因的copies/g DNA最低也能达到10

～10

之间，与北京某一养猪场周边土壤中抗性基因含量相当，有些甚至更为严重。

Abstract

Use of antibiotics at subtherapeutic concentrations for agricultural applications has been believed to be an important factor in the proliferation of bacteria with antibiotic resistance genes (ARGs). In this work

total nine tetracycline ARGs (tet-ARGs) including tetA

tetB

tetC

tetD

tetG

tetL

tetO

tetQ and tetX) were studied in sixteen soil samples from livestock using qualitative PCR SYBR Green I real-time PCR methods. The results showed that eight of tet-ARGs except tetD could be detected in most of samples and the detection percentages ranged from 6% (tetB) to 94% (tetC). The quantitative results for four tet-genes (tetA

tetC

tetG and tetX) showed that 50% samples had the highest tetC abundance (10

) and the others were in range of 10

to 10

. As a result

the spread occurrences of high levels of tet-genes in livestock soil should not be overlooked.

关键词

四环素抗性基因养殖场土壤基因污染

Keywords

tet-ARGslivestock soilgene contamination

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