Gynogenesis is the production of an embryo from an egg after penetration by a spermatozoon that does not contribute genetic material. The process of gynogenesis involves two steps: firstly

the sperm is inactivated by UV irradiation;secondly

the meiotic diploids are made by restraint of the extrusion of the secondary polar body. Gynogenesis was induced in two groups of Russian sturgeon (

Acipenser gueldenstaedtii

) by cool shock and heat shock respectively. Cool shock (M1) and heat shock (M2) gynogensis offspring were gained separately. Six polymorphic microsatellite markers were used to investigate genotype by PCR amplification. Twenty fries in each group including gynogenetic families M1

control group and their parents and forty fries in gynogenetic families M2 were chose to be analyzed .The average expected heteroaygosity (

H

e

)

allele number(

A

) and allele frequencies(

P

) in the cool shock line was 0.591

6.0 and 0.100~0.708 respectively. In the heat shock line

the average expected heteroaygosity

allele number and allele frequencies was 0.687

5.5 and 0.006~0.774 respectively. Special microsatellite bands of male parent were used as diagnostic markers to analyze the hybrid of gynogenesis offspring. The results showed that 40 % in cool shock genogenesis offspring and 27.5% in heat shock genogenesis offspring were hybrid respectively. And

it is consistent with phenotypic identification results. Haploid individuals were identified in 10% of cook shock group and 15% of heat shock group. Complete gynogenetic progenies were found only in heat shock group

and they were just about 22.5%.Gene recombination in different levels were found in other offspring except haploid

hybrid and gynogenisis offspring of two gynogenetic lines.