This study investigated the polymorphism of Region V of the mitochondrial DNA and Ychromosome DYS287 sites in 9 ethnic groups of Guizhou to provide the referable molecular genetics data with related ethnic groups of Guizhou. Polymerase chain reactionpolyacrylamide SDS gel electrophoresis and Polymerase chain reactionagarose gel electrophoresis were applied to detect the polymorphism of 9bp deletion of mitochondrial DNA noncoding region and Ychromosome DYS287 sites. Two type of polymorphism in mitochondrial DNA were discovered：standard form and deletion form; the frequency of 9bp deletion of mtDNA in Gelao、She、Zhuang、Mulao、Maonan、Menggu、Hui、Qiang、Man people were: 26.6 %、25 %、20 %、18.3 %、13.3 %、10 %、8.3 %、6.7 %、5 %

respectively; two type of polymorphism in Ychromosome DYS287：YAP

+

and YAP

-



the frequency of YAP

+

in Gelao、Maonan were: 20 %、1.67 %

respectively

others were 0.The data of polymorphism of Region V of mitochondrial DNA and Y-chromosome DYS287 sites were obtained in 9 ethnic groups of Guizhou and the genetic information were provided for ethnic groups.