Under the condition of physiological environment at pH 7.4

the binding ratio

n

γ-CD

∶

n

AYR

=1∶1 and binding constant K

Θ

f

288.15K

=1.69×10

5

L/mol were confirmed by mole ratio method and linear method. AO was used as molecule probe

and the interaction between γ-CD-AYR and herring-sperm DNA was studied by UV spectra

fluorescence spectra

thermodynamic method and viscosity method

etc. A series of thermodynamic data such as Δ

r

H

m

Θ

=8.78×10

3

J/mol

Δ

r

G

m

Θ

=-2.59×10

4

J/mol

Δ

r

S

m

Θ

=116.45 J/(mol·K) were obtained

and Δ

r

S

m

Θ

was the primary driven power of this interaction

the binding ratio was

n

DNA

∶

n

γ-CD-AYR

=1∶2

the binding constants were

K

288.15K

= 3.10×10

4

L/mol

K

310

.15K

4.02×10

4

L/mol. The interaction mode between γ-CD-AYR and herring-sperm DNA was an electrostatic interaction and part embedded intercalation.