The aim of the present study is to verify whether roflumilast attenuates PQinduced injury and fibrosis in lung

liver and kidney tissues of mice. Mice are pretreated with PQ of 20 mg/kg (ip.)

and then dealt with roflumilast or dexamethasone 5 mg/(kg·d) for 21 days. The tissues of lung

liver and kidney are surgically separated and immersed in 10% neutral formalin. The injury and fibrosis in tissues of lung

liver and kidney are observed by H&E staining and Masson staining. The results show that PQ (20 mg/kg

ip.) induces remarkable toxicity and fibrosis in tissues of lung

liver and kidney. Roflumilast (5 mg/kg per day for 21 days) treatment reduces PQinduced injury and fibrosis in tissues of lung

liver and kidney

which is superior to dexamethasone. So

roflumilast attenuates PQinduced injury and fibrosis in tissues of lung

liver and kidney of mice.