Studies of β-glucosidase enzymatic properties from

Hypocrea

sp. W

63

show that

enzyme activity determined by pNPG (4-nitrophenyl β-D-glucopyranoside) can attain to 482.1 U/mL. The optimum reaction pH value and temperature are 4.8 and 65 ℃

respectively. 10% of ethanol has the greatest promotion

the β-glucosidase activity can nearly increased double and the ethanol tolerance of β-glucosidase can attain to 30%. When simultaneous saccharification and fermentation(SSF) carried out for 120 h with β-glucosidase adjunction

41.25 g/L of ethanol can be obtained. Compared with control

the ethanol production can increase nearly 2-fold. The β-glucosidase with high enzyme activity

thermostable and alcohol tolerance for SSF application

indicate that the β-glucosidase producing strain has great potent application on cellulosic ethanol production.