Isolation, Identification and Enzyme-producing Conditions Optimization of#br#
Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite

GAO Yunhang; WANG Wei; LI Qiuju; MA Hongxia; LOU Yujie

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Isolation, Identification and Enzyme-producing Conditions Optimization of#br# Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite

更新时间：2023-12-11

- Isolation, Identification and Enzyme-producing Conditions Optimization of#br# Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 52, Issue 2, Pages: 84-89(2013)
- 作者机构：
  
  1. 吉林农业大学动物科技学院,吉林,长春,130118
  2. 吉林农业大学动物生产与产品质量安全教育部重点实验室,吉林,长春,130118
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013，
  
  Published Online：25 March 2013，
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GAO Yunhang, WANG Wei, LI Qiuju, et al. Isolation, Identification and Enzyme-producing Conditions Optimization of#br# Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 52(2):84-89(2013)
DOI：

GAO Yunhang, WANG Wei, LI Qiuju, et al. Isolation, Identification and Enzyme-producing Conditions Optimization of#br# Lignin-Utilizing and Cellulose-Utilizing Bacterium from the Gut of Termite. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 52(2):84-89(2013) DOI：

摘要

利用刚果红法、Azure-B平板法从白蚁肠道中分离出5株同时具有木质素降解和纤维素分解功能的菌株，选取其中分解功能最强的菌株MX5经形态观察、生化鉴定和16S rRNA鉴定为芽孢杆菌属的地衣芽孢杆菌

Bacillus licheniformis

。产酶条件优化试验结果表明，菌株MX5以

=0.5%秸秆为碳源

=0.5%酵母粉和硫酸铵混合物为氮源，初始pH8.0，37 ℃摇瓶培养96 h，接种量为1%时，产酶活性最高。筛选出产酶活性优良的菌株，对提高木质纤维素的利用率、降低环境污染等方面意义深远。

Abstract

Five strains of bacteria with decomposed function for lignin and cellulose were isolated from gut of termite with a congo red and Azure-B plate method. MX5 strain with the strongest decomposed function was identified as Bacillus licheniformis by morphological observation

physiological and biochemical identification and 16S rRNA identification. Optimization test of producing lignin and cellulose show MX5 strain has the highest production activity with 0.5% of straw as carbon source

mixture of 0.5% yeast extract and ammonium sulfate as the nitrogen source and cultrue in shake flask at 37 ℃ for 96 h with 1%noculation amount and initial pH 8.0. Selecting excellent strains which produced ligninase and cellulase has far-reaching significance for improving the utilization of wood fiber feed

reducing environmental pollution and so on..

关键词

白蚁木质素分解菌纤维素分解菌鉴定条件优化

Keywords

termitelignin decomposing bacteriacellulose decomposing bacteriaidentificationconditions optimize

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College of Animal Science and Technology, Jilin Agricultural University

Key Laboratory of Animal Production, Product Quality and Security, Jilin Agriculture University, Changchun

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