The rapidly developing molecular techniques require the ability to extract high quality RNA from Aspergillus flavus. The cell wall structure and endogenous RNase activity in A. flavus are often the main obstacles for obtaining RNA with high quality. In this paper

TES hot phenol method was applied to obtain total RNA from A. flavus

and the total RNA was further used in RT-PCR (Reverse translation-polymerase chain reaction) and RNA-sequencing.The results showed that the TES hot phenol method can be used to get a good quality of total RNA from this filamentous fungus as templates for following studies

such as RT-PCR and RNA sequencing.