High Cell Density Culture, Purification and Identification of Algicidal Components of An Indigenous Algicidal Bacteria L7

YANG Lili; PAN Weibin; WEN Yong; CHEN Qun

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High Cell Density Culture, Purification and Identification of Algicidal Components of An Indigenous Algicidal Bacteria L7

更新时间：2023-12-11

- High Cell Density Culture, Purification and Identification of Algicidal Components of An Indigenous Algicidal Bacteria L7
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 52, Issue 1, Pages: 83-88(2013)
- 作者机构：
  
  1. 华南理工大学环境科学与工程学院,广东,广州,510640
  2. 环境保护部华南环境科学研究所,广东,广州,510655
  3. 广东省建筑科学研究所,广东,广州,510500
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013，
  
  Published Online：25 January 2013，
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YANG Lili, PAN Weibin, WEN Yong, et al. High Cell Density Culture, Purification and Identification of Algicidal Components of An Indigenous Algicidal Bacteria L7. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 52(1):83-88(2013)
DOI：

YANG Lili, PAN Weibin, WEN Yong, et al. High Cell Density Culture, Purification and Identification of Algicidal Components of An Indigenous Algicidal Bacteria L7. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 52(1):83-88(2013) DOI：

摘要

针对一株溶藻细菌L7，探索溶藻细菌高密度培养的工艺参数及溶藻活性物质提纯鉴定的关键技术，为生物杀藻剂的研制奠定理论基础。在溶藻细菌L7的高密度培养阶段，通过设置单因素实验及正交实验、应用摇瓶及自动发酵罐筛选出适宜培养溶藻细菌L7的培养基（碳源葡萄糖、氮源氯化铵、C/N质量比3∶1、初始pH值7.5）、细菌接种量3.1×10

cfu/mL、DO［30%（±10%）］及搅拌速率［(160±10)r/min］；在溶藻细菌L7溶藻活性物质的提纯鉴定阶段，通过透析、凝胶柱层析、高效液相色谱、液质联用仪等物质提纯鉴定手段，获得2种溶藻活性物质，相对分子质量分别为588.2、365.0，相较而言，相对分子质量为365.0的物质的溶藻作用更强。

Abstract

Base on an indigenous algicidal bacteria L7

technical and process parameters for high cell density culture and the research and development of biological algicide were investigated. In the stage of high cell density culture

we selected the appropriate cell culture medium of bacteria L7 (carbon and nitrogen source are glucose+NH

C/N ratio is 3∶1

pH 7.5)

bacterial load (3.1×10

cfu/mL)

DO (30%（±10%）) and agitation speed (160(±10) r/min) through the setting of single factor experiment and orthogonal experiment

the application of shake flask and automatic fermentor. The process of purify and identify algicidal components of L7 included dialysis

gel chromatography

HPLC

MS and et al. Finally two algicidal components were received

the molecular weights were 588.2 and 365.0 respectively

and the algicidal effect of the latter one weas better.

关键词

溶藻细菌溶藻活性物质高密度培养提纯鉴定水华鱼腥藻

Keywords

algicidal bacteriaalgicidal componenthigh cell density culturepurification and identificationAnabaena flos-aqua

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