The application prospect in sea water fish breeding influenced by the inclined Epinephelus coioides recombinant proteins was studied. The supernatant containing recombinant protein was sprayed by concentration of 2

4

8 milligrams per Kilogram basic orangespotted grouper diet

and the single factor experiment was composed with one control group（G0）and three treated groups（G1，G2，G3）. The fishes were raised in circulating water systems indoor and the experiment contained 3 replicates in each group. The feeding period was 10 weeks

and in this period the fishes were fed three times every day

morning

mid-noon and afternoon respectively. The water quality was monitored regularly. Results showed as follows，the G2 group possessed the highest weight gain rate for 293.16%

and  23.75% higher than control group

but its feeding rate was 1.14

which was the lowest in all groups; The length gain rate and average feeding amount of G2 group was significantly higher than that of control group (p<0.05) . The crude protein of G2 group was significantly higher than control group (p<0.05) in the fish bodies

but the muscle crude lipid was significantly lower than control (p<0.05). The treatments cholesterol

low density lipoprotein and the triglyceride in serum were decreased compared with control

and showed significant differences between G1

G2 group and G0 group (p<0.05). The amylase activity in treatments was higher than control

and the G2 group showed the significant difference (p<0.05); the lipase activity of treatments was significantly higher than control (p <0.05).The total antioxidant capacity (T-AOC) of G2 group was the highest and significantly higher than control (p<0.05); the catalase activity of G3 group was significantly higher than control (p<0.05); the treatment groups MDA content was decreased than control. The phosphates activity of G2 group was the highest in all groups and was significantly higher than control (p<0.05) in liver. The above results showed that it had an obvious promoting effect if added 4mg/kg of growth hormone gene recombinant proteins to the feed and could improve the utilization rate of feed

besides

the blood lipid level of Epinephelus coioides was reduced obviously if added appropriate growth hormone gene recombinant protein and could improve its ability to digest fat and antioxidant capacity.