CVNH (cyanovirin-N homology

CVNH) is the homology protein of CVN

which is a novel anti-HIV glycoprotein. In the previous study

we have firstly reported the full-length sequences of CVNH gene cloned from

Ceratopteris thalictroides

and constructed prokaryotic expression plasmid pET32a-CtCVNH. The plasmid DNA was transformed into Rosetta 2 (DE3)

E. coli

cells for expression of the recombinant protein. In the study

we optimized culture conditions of CVNH protein expression

including the types and ingredients of the culture medium

initial pH

A

600nm

value

induction agents and concentrations

induction time

and expression time

respectively. The optimized culture conditions contained TB medium with 24 g·L

-1

yeast extract and 72 g·L

-1

tryptone

initial pH 8.0

and lactose induced for 6 hours at A

600nm

0.6

which would increase CVNH productivity to 1.9 times than before. This result laid a foundation to explore the drug development of CNVN.