Bacillus thuringiensis is widely used for pest control. However

the fermentation production of this bacterium was severely influenced by the ramdom outbreak of lysogenic phage from B. thuringiensisIn the present study

a novel bacteriophage MZTP02 was isolated from industrial 〖WTBX〗B. thuringiensis strain MZ1 and sequenced for tmp gene encoding tail tape measure protein. By assisting and control the tail packaging of bacteriophage

the protein is an essential component to help phage attach to the host for successful infection. By PCR

tmp gene was amplified from the genome DNA of MZTP02 and cloned into pQE30 and expressed in M15. The TMP protein of 35 000 was obtained and purified by Ni-NTA column. Using the purified TMP protein as the immunogen

polyclonal serum was raised in the New Zealand rabbit. Western blot analysis of TMP proteins expressed in prokaryotic cells demonstrated a polypeptide with size of 35 000 which was in agreement with the predicted size of this protein. Results of plaque formation of phage after TMP antiserum treatment showed a negative relationship to the concentration of antiserum when the concentration of multiplicity of infection (m.o.i) was less than 1

e.g. the titers were 1×10

5

pfu/mL and 2×10

3

pfu/mL when a degnized amounts of phage were mixed with TMP antiserum with concentration of 25 μg/mL and 250 μg/mL respectively. This result indicated that TMP antiserum can combine with TMP protein

and then depress the phage titer.