The effect of reaction temperature and pH on the activities of 3 main digestive enzymes (protease

lipase

amylase) of Cromileptes altivelis were studied using enzymology analysis method. The results show that: the activities of all digestive enzymes in Cromileptes altivelis increased at first and then decreased with temperature and pH increasing in the designed temperature and pH ranges. In stomach

the optimal temperature for protease was 40～45 ℃

whereas all in intestine

pyloric caecum and liver were 40 ℃. The optimum temperature for lipase in stomach was 35 ℃

the optimum temperature for the pyloric caecum was 40～45 ℃

the others were all 40 ℃ respectively. The optimum temperature for amylase in stomach was 40 ℃

The others were all 35 ℃ respectively. The optimum pH for protease in stomach was 3.2

the optimum pH values in intestine

pyloric caecum and liver were all 82.The optimum pH values for lipase in stomach and liver were 7.2

8.2

the others were all 6.2 respectively The optimum temperature for amylase in stomach was pH 7.2～8.2 and others were all 7.2Under optimum temperature and pH

the activities of lipase in different organs was in the order of anterior intestine> stomach> liver> pyloric caecum

and the activities of amylase were intestinal> the pyloric caecum> stomach> liverUnder optimum temperature and pH

the order of protease was intestine> stomach> liver> pyloric caecum and intestine> stomach> pyloric caecum> liver