Application AQC as a Pre-column Derivatization Reagent for HPLC Determination of Free Amino Acids in Borojo sorbilis Cuter.

LIU Bo; HAN Xiaodong; ZHANG Minting; XU Fangfang; ZHOU Wen; JIAO Hong

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Application AQC as a Pre-column Derivatization Reagent for HPLC Determination of Free Amino Acids in Borojo sorbilis Cuter.

更新时间：2023-12-11

- Application AQC as a Pre-column Derivatization Reagent for HPLC Determination of Free Amino Acids in Borojo sorbilis Cuter.
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 52, Issue 4, Pages: 100-104(2013)
- 作者机构：
  
  1. ．广州中医药大学第二附属医院,广东,广州,510006
  2.  2．广东出入境检验检疫局,广东,广州,510623
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2013，
  
  Published Online：25 July 2013，
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LIU Bo, HAN Xiaodong, ZHANG Minting, et al. Application AQC as a Pre-column Derivatization Reagent for HPLC Determination of Free Amino Acids in Borojo sorbilis Cuter.. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 52(4):100-104(2013)
DOI：

LIU Bo, HAN Xiaodong, ZHANG Minting, et al. Application AQC as a Pre-column Derivatization Reagent for HPLC Determination of Free Amino Acids in Borojo sorbilis Cuter.. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 52(4):100-104(2013) DOI：

摘要

为了测定宝乐果中游离氨基酸的种类以及含量。该文的样品用6-氨基喹啉-N-羟基琥珀酰亚胺基氨基甲酸酯(AQC)柱前衍生后进行高效液相色谱(HPLC)分析。采用Phenomenex Luna C

(250 mm × 4.6 mm

5 μm)色谱柱，柱温为35 ℃；流动相A为去离子水，流动相B为乙腈，流动相C为10 mmol/L醋酸铵缓冲液(以冰醋酸调至pH 5-10)，梯度洗脱，流速为0.8~1.0 mL/min；检测波长为248 nm，同时测定宝乐果中的游离氨基酸。结果得：18种氨基酸在0.02~0.5 mmol/L浓度范围内有良好的线性关系，r 均在0.998 3~0.999 2之间；宝乐果中测得14种游离氨基酸，其含量范围为0.087~2.680 mg/g，平均加样回收率为96.9 %~106.9 %，RSD为0.67 %~3.07 %。该方法可用于检测宝乐果中游离氨基酸含量，测定的结果为宝乐果质量研究和评价提供了依据。

Abstract

To determine and analyze free amino acids in Borojo.The sample was derivatized with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) and then was analyzed with highperformance liquid chromatography (HPLC). HPLC was performed on a Phenomenex C

(250mm × 4.6 mm

5 μm) column with gradient elution of deionized water (A)

acetonitrile (B) and 10 mmol/L ammonium acetate buffer solution(adjusted to pH 5-10 with glacial acetic acid) (C) at the flow rate of 0.8~1.0 mL/min

detected with Ultra Voilet (UV) at 248 nm. Linear relationship equations were obtained with 18 kinds of amino acids in the range of 0.02~0.5 mmol/L

and their correlation coefficients were in the range of 0.998 3~0.999 2. Fourteen amino acids were detected and average recoveries of those added were in the range of 96.9 %~106.9 % and RSD values were in the range of 0.67 %~3.07 %. The method could be used for determination of content of free amino acids in Borojo.

关键词

宝乐果AQC游离氨基酸柱前衍生法

Keywords

BorojoAQCfree amino acidspre-column derivatization

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