In order to screen the efficient cold-adapted cellulose-decomposing strains and optimize conditions for maximum production of enzyme activity， in this study

we isolated the cold-adapted cellulose-decomposing strains with congo red method and flask fermentation from cattle manure and the soil of mountain. The strains were identified by morphological observation and molecular biology identification

and their fermentation condition were also optimized. The result showed that their FPA were (11.23±0.39) U/mL

(5.59±0.36) U/mL

and the strain FF2-2 and F-3I were respectively identified as Aureobasidium pullulans and Aspergillus versicolor. The optimum carbon and nitrogen source of two strains were 0.5 % wheat bran

0.5 % starch

and 1 % beef extract and ammonium sulfate mixture

1 % beef extract

respectively. The optimum pH were 7.0 and 6.0

respectively. The optimum fermentation temperature for both strains was 23 ℃

and the optimum fermentation time was 3 d

5 d respectively. The FPAs of strain FF2-2 and F-3I increased 2.6 and 5.6 times respectively after optimization.