Ethidium bromide（EB）was used as molecule probe

the interaction between β-cyclodextrin-4-Benzyloxypheno (β-CD-PBP) and herring-sperm DNA was studied in physiological buffer solution (pH=7.4) by fluorescence spectroscopy

thermodynamic analysis and viscosity method. Based on the mole ratio method and linear method

the parameters were determined as follows: the binding ration

β-CD

: n

PBP

 =1 : 1 and binding constantK

f

= 7.39×10

3

L·mol

-1

. The binding ratio was n

DNA

: n

β-CD-PBP

 =1 : 9

the thermodynamic perameters were Δ

r

H

m

θ

＝1.44×10

5

J/mol，Δ

r

G

m

θ

300.15K

＝－1.97×10

4

J/mol，Δ

r

S

m

θ

＝549.32 J/(mol·K)

 Δ

r

S

m

θ

was the primary driven power of this interaction

the binding constants were K

300.15K

=2.86×10

3

L/mol，K

310.15K

=2.69×10

4

L/mol. These results indicated that the interaction between β-CD-PBP and herring-sperm DNA belonged to electrostatic and part intercalation.