To continue to study anti-tumour bioactive saponins EtOAc layer from methanol extract of the stem of psychotria sp.

four compounds were obtained by all kinds of column chromatography

such as silica gel

Sephdex LH-20

and ODS. Their structures were characterized by spectroscopy including NMR

MS

GCMS analyses

and chemical and the physical methods. The result indicated that they were Psychotrianosides G (1)

Ardisianosides D (2)

β-amyrin (3)

and Stigmasterol3-O-β-D glycosidase(4). Cyclamiretin A (5) was obtained by acid hydrolysis of 2. Psychotrianosides G(1) was firstly obtained as a natural product. The chemical structure of compound (1) was analyzed by 2D NMR firstly. Compounds 1

2

3

4

5 were firstly obtained from genus of psychotria.