The purpose of study is to establish a method to improve the ethanol output by saccharification and fermentation of food waste in an order to realize the industrialization in the future. By the way of combining two or three kinds of enzymes to obtain an optimization

it was shown that positive synergism effect was observed between glucoamylase (120 U/g) and isoamylase (300 U/g)

and the glucose concentration in the hydrolysate reached 130.29 g/L. Simultaneous saccharification and fermentation (SSF) and separate hydrolysis and fermentation (SHF) of food waste have been conducted using Saccharomyces cerevisiae CICC 1346 and 120 U/g of glucoamylase and 300 U/g of isoamylase. The yields of ethanol in SSF and SHF were 49.48 and 45.89 g/L

and the conversion rates from glucose to ethanol were 91.7% and 77.1%

respectively. Compared to the SHF process

the SSF one was easier to operate and showed shorter fermentation period but higher ethanol production rate

which makes it more suitable for industrial application.