Vitro Properties Invesigation of Histidylated PEG-b-PLG-g-PEIs As Gene Carrie

BAI Feng; TIAN Shouqing; FENG Ming

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Vitro Properties Invesigation of Histidylated PEG-b-PLG-g-PEIs As Gene Carrie

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- Vitro Properties Invesigation of Histidylated PEG-b-PLG-g-PEIs As Gene Carrie
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 54, Issue 3, Pages: 107-114(2015)
- 作者机构：
  
  中山大学药学院,广东,广州,510080
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2015，
  
  Published Online：25 June 2015，
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BAI Feng, TIAN Shouqing, FENG Ming. Vitro Properties Invesigation of Histidylated PEG-b-PLG-g-PEIs As Gene Carrie. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 54(3):107-114(2015)
DOI：

BAI Feng, TIAN Shouqing, FENG Ming. Vitro Properties Invesigation of Histidylated PEG-b-PLG-g-PEIs As Gene Carrie. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 54(3):107-114(2015) DOI：

摘要

为了对组氨酸修饰的阳离子聚合物载体进行研究

重点考察其基因转染效率与细胞毒性，探讨其作为基因载体的可能性。采用自由基聚合法合成聚乙二醇-聚谷氨酸-聚乙烯亚胺-组氨酸(PEG-b-PLG-g-PEIs-HISs

GGIS)聚阳离子载体，用

H-NMR 表征载体材料结构；通过凝胶电泳实验研究载体对质粒DNA的压缩能力，粒径分析仪测定载体结合DNA后在不同N/P比条件下的粒径及表面电荷；用MTT法测定载体在HEK293T

Hela

BEL7402和A549等细胞株上的细胞毒性，考察GGIS体外细胞转染效率。结果显示在N/P≤30时，载体材料的平均粒径在100~200 nm，表面电荷在10~30 mV，适合细胞吞噬。体外细胞毒性表明，GGIS的细胞毒性较PEI 25K低。GGIS具有较强的DNA缩合能力，且有较好的体外基因转染能力。因此，GGIS有较好的体外基因转染能力，能够携带报告基因在体外有效表达，是具有应用前景的非病毒性基因药物载体。

Abstract

The study mainly focuses on cell transfection efficiency and cytotoxicity of a histidylated cationic copolymer in order to explore its potential as a gene carrier. A novel histidylated cationic copolymer，PEG-b-PLG-g-PEIs-HISs(GGIS)

was synthesized by free radical polymerization．The chemistry structure of GGIS was confirmed by

H-NMR.Agarose gelelectrophoresis was performed to study the capacity of plasmid DNA condensation. Particle size and zeta potential were measured at various N/P ratios. Cytotoxicity of GGIS was determined by MTT assay in HEK 293T

Hela

BEL7402 and A549 cells lines .The transfection efficiency of GGIS was specifically evaluated and monitored by flow cytometry. When the N/P less than of 30. The size of the DNA/GGIS was approximately 100～200 nm and the zeta-potential was 10~30 mV.The MTT assay showed that the cytotoxicity of GGIS was obviously lower than PEI 25K. GGIS demonstrated significantly higher transfection efficiency than PEI 25K.Therefore

GGIS with high transfection efficiency and low cytotoxicity has great potential to be used as non-viral gene carriers．

关键词

基因治疗聚乙烯亚胺聚谷氨酸组氨酸基因传递转染效率

Keywords

gene therapyPEIpoly(β-benzyl-L-glutamate)histidinegene deliverygene transfection

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