Many zinc finger units

which can specifically recognize and bind to DNA

are strung together to form zinc finger protein domain. There are many uses to artificially construct the protein molecules

such as various site modification or regulation of the genome

by its integration with the different functional domains of active factors. This study  focused on fibroin heavy chain gene of silkworm. Firstly

a suitable target site was found using Zinc Finger Tools software

and then we used module assembled technology to construct a pair of four zinc finger domain targeting the specific site of fibroin heavy chain gene

through restriction enzyme digesting

fragment recycling

linking

transforming

and other steps. The results would provide a good foundation for the fixedpoint modification or reconstruction of fibroin genes to improve silk quality and production

or to greatly enhance the expression of exogenous protein in the bioreactor of Bombyx mori.