Extraction and antioxidant activity of Proanthocyanidins from Spatholobus suberectus Dunn

DONG Pan; LUO Zexin; WANG Dongmei

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Extraction and antioxidant activity of Proanthocyanidins from Spatholobus suberectus Dunn

更新时间：2023-12-11

- Extraction and antioxidant activity of Proanthocyanidins from Spatholobus suberectus Dunn
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 56, Issue 1, Pages: 8-13(2017)
- 作者机构：
  
  中山大学药学院,广东,广州,510006
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2017，
  
  Published Online：25 January 2017，
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DONG Pan, LUO Zexin, WANG Dongmei. Extraction and antioxidant activity of Proanthocyanidins from Spatholobus suberectus Dunn. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 56(1):8-13(2017)
DOI：

DONG Pan, LUO Zexin, WANG Dongmei. Extraction and antioxidant activity of Proanthocyanidins from Spatholobus suberectus Dunn. [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 56(1):8-13(2017) DOI：

摘要

在单因素试验的基础上，运用响应面法优化鸡血藤 Spatholobus suberectus Dunn 原花青素的超声波辅助提取工艺。以料液比、丙酮体积分数、提取时间为考察因素，以原花青素的提取率为响应因子，进行Box-behnken中心组合试验，得到最佳提取工艺参数为：室温下，超声功率500 W，提取时间30 min，料液比1∶45 (m/V)，丙酮体积分数70%，提取1次。在此条件下

鸡血藤原花青素提取得率为25。59%。制得的原花青素对DPPH·、ABTS·的IC

分别为8.42

10.55μg/mL。

Abstract

Ultrasonic-assisted extraction (UAE) was used to extract proanthocyanidins (PC) from Spatholobus suberectus Dunn. Response surface methodology (RSM)

based on a three-factor

three-level Box-Behnken central composite design (BBD)

was employed to obtain the optimal extraction parameters

in which the extraction yields of PC was maximum. Three important factors

including extraction time

solidliquid ratio and acetone concentration

were selected by single-factor tests. Optimized extraction conditions for PC were a ratio of liquid to solid of 1∶45 mL/g

25 ℃

30 min

and acetone concentration as 70%

one-time extraction

500 W ultrasonic power. Under these conditions，the extraction yield of PC was 25.58%. In DPPH· and ABTS· scavenging assays

the IC

of PC from S. suberectus were 8.42 μg/mL and 10.55 μg/mL

respectively.

关键词

鸡血藤Spatholobus suberectus Dunn原花青素响应面法抗氧化活性

Keywords

Spatholobus suberectus Dunnproanthocyanidinsresponse surface methodologyantioxidant activity

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