In order to reveal the effects of dissolved matters of eucalyptus leaves to the aquatic environments. Solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GCMS) was selected for qualitative and quantitative determination of dissolved matters of eucalyptus leaves in pure water during different immersing times in this study. By searching NIST database

139 different chemicals are identified with the matching degree higher than 85%

and the composition of dissolved matters varies significantly during the immersing time. While using SPME as the sample pretreatment method

the identified chemicals are much more than those reported previously. Moreover

22 chemicals with relative abundance higher than 1% and the detection rate higher than 80%

are investigated quantitatively. The result shows that 7 components increase by the increasing immersing times

and reach the highest concentration of 5 848 ng/mL after 30 days. Nevertheless

the COD and TN values of the dissolved matters are inferior to Ⅴ class of surface water quality standards of China

while NH

+

4

-N is inferior to Ⅲ class after 15 days. Obviously

more attentions should be paid for the effects of dissolved matters of eucalyptus Leaves to the aquatic environments.