This study was to investigate the time phase of circadian rhythm of advances of light/dark cycle model mice

the effect of electro-acupuncture (EA) on their activity rhythms and the molecular regulating mechanism on the clock genes of suprachiasmatic nucleus (SCN). 44 male C57BL/6J mice were divided into 4 groups which were the blank group(n=10)

the model group(n=12)

the binding group(n=12) and the electroacupuncture group (n=10) . We housed mice in LD cycle for 10 days. The control group was kept 12 h∶12 h LD of 20 days

and then SCN collected at ZT18. Then 8 hours advance of light onset every 2 days of 5 times. The model group was collected tissue at ZT18 when model successful. The binding group binds as the same time with the electro-acupuncture group. Electro-acupuncture group was treated at ZT16

on Zhiyang (GV 9) and Ganshu (BL 18) with acupuncture needle one time a day of three days. The SCN were collected at ZT18 at the third day. At last

samples of SCN were measured by PCR Array method to test the gene expression level. The experiment results are：① After the model was established

phase positions and onset of activity rhythms were advanced and period was shorten compared with the control group (P<0.05). After resynchronization

the phase positions and onset of activity rhythms of the electro-acupuncture group were delay respectively compared with the control group and the model group. The period at the 1-st and 2-nd day after resynchronization of the electro-acupuncture group had significant differences with the control group and itself before.② Compared with the control group

there were 8 genes upregulated(Aanat、Crx 、Epo、Nkx2-5、Pax4、Prf1、Rora、Stat5a) and 4 genes decreased(Egr1、Per1 、Per3、Prokr2) of the model group. There were 3 genes upregulated(Esrra、Mat2a、Per3) and 11 genes decreased(Cartpt、Crx、Epo、Kcnma1、Mtnr1b、Nkx25、Nms、Pax4、Prf1、Prkacb、Prkca) of binding group compared with model group; after electroacupuncture there were 6 upregulated(Egr1、Esrra、Mat2a、Per1、Per3、Prokr2) and 21 decreased(Aanat、Arntl、Cartpt、Crx、Csnk1e、Epo、Kcnma1、Mtnr1b、Myod1、Nkx25、Nms、Opn3、Pax4、Prf1、Prkacb、Prkca、Prkcb、Prokr2、Rora、Rorb、Slc9a3、Tgfb1). The results indicate electroacupuncture can regulate onset of activity rhythms and period. Regulating the expressions of some clock genes in SCN of the circadian rhythm disturbance mice was probably one of the molecular mechanisms of electroacupuncture to regulate the activity rhythms of circadian rhythm disturbance animals.