The aim of this study was to select the effective microbial agents to remediate heavy metal pollutants by investigating the potential of the growing biomass of the three endophytic fungi through bioaccumulation to remove  metals from contaminated water. In this study

 three endophytic fungi Fusarium sp. CBRF14

 Penicillium sp. CBRF65

 Alternaria sp. CBSF68 tolerant Cd

Pb

and Zn were isolated from rapes growing in DaBao metal contaminated soils. And then we investigated the effects of initial metals (Cd

Pb and Zn) concentrations on the biomass

the metal bioaccumulation capacities and bioaccumulation percentages of the endophytic fungi. The results showed that the bioaccumulation percentages of Cd and Zn by CBRF14

CBRF65 and CBSF68 decreased with the increase of initial concentrations of Cd and Zn in general

however

the bioaccumulation percentage of Pb by CBRF14 and CBSF68 showed a pattern of “increase-decline”. With the increase of the initial Cd

Pb

and Zn concentrations

the biomass of CBRF14

CBRF65 and CBSF68 showed the declined trend

but the bioaccumulation capacities of metals increased. The maximum bioaccumulation capacities of CBRF14

CBRF65 and CBSF68 were 20.5~53.4 mg·g

-1

for Cd

188.9~356.6 mg·g

-1

for Pb

and 28.4 ~ 292.8 mg·g

-1

for Zn

respectively. The results indicated that the three heavy metal-resistant endophytic fungi from rapes could live and accumulate metals in solutions with heavy metals

which suggested that they had the potential to remove heavy metals from metal-contaminated water. The results also indicated that plants accumulating multiple metals might harbor diverse fungal endophytes suitable for bioaccumulation of metals.