Haemocoagulase Acutus (Halase)

a new hemostatic in development

is a highperformance thrombin-like enzyme (TLE) derived from snake venom of Agkistrodon acutus. Here we reported a new process established for separation and purification of Halase from snake venom. Combined with tangential flow filtration

  ion exchange chromatography was conducted twice to effectively separate Halase

and two gel exclusion columns were used to remove pyrogen and salt. This new method is simple and efficient

and generates a high purity of Halase

making it a promising process in industrial scale application.