N16 protein is a potential agent for treatment of osteoporosis due to its favorable anabolic and antiresorptive effects on bone. Our work described a biological engineering method to prepare N16. To build the high level expression system

pRHis plasmid was transformed into BL21 (DE3) plysS strain. The present study has established a complete N16 preparation process with simple protein expression and purification procedures. This procedure can improve N16 protein production

reduce the cost and shorten the cycle

which will facilitate further studies on the functions of N16.