Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath)

LIANG Ziqiang; LIANG Shike; LIU Tingting; LI Guanghong; WANG Fanghai

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Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath)

更新时间：2023-12-11

- Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath)
- Acta Scientiarum Naturalium Universitatis SunYatseni Vol. 56, Issue 1, Pages: 121-124(2017)
- 作者机构：
  
  中山大学有害生物控制与资源利用国家重点实验室//昆虫学研究所,广东,广州,510275
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2017，
  
  Published Online：25 January 2017，
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LIANG Ziqiang, LIANG Shike, LIU Tingting, et al. Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 56(1):121-124(2017)
DOI：

LIANG Ziqiang, LIANG Shike, LIU Tingting, et al. Clonging and phylogenetic analysis of 18S rDNA gene from Sogatella furcifera (Horvath). [J]. Acta Scientiarum Naturalium Universitatis SunYatseni 56(1):121-124(2017) DOI：

摘要

以白背飞虱Sogatella furcifera长翅雌虫为材料，提取其基因组DNA，以已知的半翅目昆虫18S rDNA全序列和白背飞虱18S rDNA部分序列为参考，通过PCR扩增出白背飞虱18S rDNA的未知部分序列，最后通过拼接，首次得到了白背飞虱18S rDNA的全长序列，长度为1 891 bp，碱基组成比例均衡，分别为：24.1% A；23.7% C；27.7% G；24.5% T。将此全长序列与分属6个目（半翅目、双翅目、膜翅目、直翅目、鞘翅目、等翅目）昆虫的18S rDNA序列进行比较，一共发现有4个保守区域，其中第二个区域发生插入或缺失的情况最少。以18S rDNA第二保守区域进行系统发育分析，发现原同翅目昆虫多数均与半翅目昆虫菜蝽亲缘关系较近，但原同翅目飞虱科昆虫，如白背飞虱、褐飞虱则与半翅目昆虫菜蝽的亲缘关系较远，表明近年来将原同翅目昆虫全部划归为半翅目还有待商榷之处。

Abstract

The long wing female adults of Sogatella furcifera were selected to extract their genomic DNA. According to the complete 18S rDNA gene sequence from Hemiptera

the unknown 18S rDNA sequence of S. furcifera was amplified by PCR. The complete 18S rDNA gene sequence of S. furcifera was obtained for the first time by splicing the unknown and known 18S rDNA sequences

whole sequence length was 1 891 bp. Comparing 18S rDNA sequence from S. furcifera and those from other insects

four conserved regions were found and the second region had the least insertion or deletion. Based on the second region

phylogenetic analysis was made and the results showed that most Homoptera insects used in this study had a close relatives with Eurydema maracandica (Hemiptera) except Delphacida.

关键词

核糖体18S rDNA白背飞虱

Keywords

Key words：ribosome18S rDNASogatella furcifera

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Related Institution

State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yatsen University, Guangzhou

State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yatsen University, Guangzhou

Jingchu University of Technology

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The United States Department of Agriculture, ERRC， Philadelphia

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