The aim of the study was to establish an HPLC-MS/MS method for simultaneous determination of naringin and naringenin in human plasma. Naringin and naringenin were extracted from plasma by ethyl acetate

then determined by HPLC-MS/MS taking isoquercitrin as internal standard (IS). Chromatographic separation was carried out on a Agilent Poroshell 120 EC-C

18

column (3.0 mm×30 mm，2.7 μm). The mobile phase was composed of solvent A (0.1% formic acid

V/V) and B (methanol with 0.1% formic acid

V/V). The flow rate was 0.4 mL·min

-1

. Electrospray ionization (ESI) source was applied and operated in multiple reaction monitoring (MRM) mode with the transitions of m/z 579.1/270.8

m/z  270.9/150.7

m/z  463.1/2998 for naringin

naringenin and IS

respectively. The method was validated over the concentration range of 0.251 0~100.4 ng·mL

-1

for naringin

and 0.503 0~201.2 ng·mL

-1

for naringenin

respectively. The intra- and inter-run precisions were within the acceptable range

and the extraction recoveries were satisfactory. The established method is highly sensitive

accurate and reproducible

which can be applied for the simultaneous determination of naringin and naringenin in human plasma.