In order to mimic the in vivo 3D micro environment and compare proliferation rate of adipose derived stem cells (ADSCs) on 2D and 3D aligned nanofiber sheets

3D aligned poly(caprolactone) (PCL) fiber scaffolds were prepared by improving the collector for electrospinning. The fibers were around capillaries in diameter of 300 μm

which were aligned very well and with fiber diameter in the range of 300~400 nm. After surface modification

ADSCs were seeded and it was found these cells were spread very well. Regardless 2D or 3D scaffolds

on aligned fibers cells were nearly all aligned along the fiber direction. When compared with that on 2D membranes

cells proliferated faster on 3D fiber scaffolds

showing the higher potential of 3D fiber scaffolds to be applied for tissue engineering applications.