The Surflex-Dock method was adopted to predict the compound-protein target association between Danhong Injection (DHI) components and anti-thrombotic activity related targets. The Cytoscape software was used to construct the DHI compound-target network

and the ClueGO plugin was used to analyze the signaling pathway of the targets. Through virtual screening

2 028 interactions between 60 DHI components and 106 anti-thrombotic targets were obtained

and the compound-target network of DHI was established. The targets closely associated DHI components including F2

F13A

SERPINC1

PGH2

ACE

REN

PLAU and PROC，which were involved in the blood coagulation

fibrinolysis

endothelial function

vasoconstriction/dilation systems. The salvianolic acids showed more associations with anti-thrombotic targets indicating that they might be the main anti-thrombotic activity components in DHI. This study provides a basis for the further study of the multi-component and multi-target molecular mechanism of DHI.