To determine polymorphic features of 25 Y chromosome Short Tandem Repeats (Y-STR ) loci in the Zhuang ethnic and their differences from the corresponding features in other ethnics in the Guizhou province

we analyzed the 25 Y-STR loci in the Zhuang ethnic population to obtained genetic polymorphism of the ethnic group. We amplified 25 Y chromosome Short Tandem Repeats loci from the DNA samples of 67 people of Zhuang ethnic using an Y27 Plex fluorescence detection kit

followed by electrophoresis with a Genetic Analyzer. Genotyping results were analyzed with a series of genetic software

including Arlequin 3.5

Mega 7.0

and SPSS19.0. Two doublecopy loci DYS385ab and DYS387S1 were found to have 8

10 alleles

whereas the remaining 23 single-copy loci were found to have 86 different kinds of alleles with the allele number varying from 2 to 7

the allele frequency ranging from 0.014 9 to 0.970 1

and the genetic diversity varying from 0.058 8 (for DYS456) to 0.823 6 (for DYS387S1). A total of 36 haplotypes were detected

of which 66.7% were unique. The haplotype diversity was 0.957 9. The R

st

genetic distance matrix showed that the genetic structure of the paternal network of the Zhuang ethnic group in Guizhou was similar to that of the Fujian She ethnic and Inner Mongolia Mongolian. The polymorphism of the 25 Y-STR loci in Guizhous Zhuang ethnic group differed significantly from that of the Yunnan Hani

Xinjiang Uygur

Liaoning Manchu

Jilin Korean and Salzburg

with the greatest difference from that of the Xinjiang Kazak

Amdo Tibetan

and West African people. The 25 Y-STR loci in the Guizhou's Zhuang ethnic group had great genetic polymorphisms

which can be used in the identification of parental relationship.